Background:

Novel human Ste20-related kinase Mst4 is biologically active in the activation of MEK/ERK pathway and in mediating cell growth and transformation. It is pro apoptotic and is highly expressed in placenta, thymus, and peripheral blood leukocytes. Interaction with Golgi matrix protein GOLGA2 results in autophosphorylation on Thr-178, possibly as a consequence of stabilization of dimer formation. This may also be activated by C terminal cleavage. MST3 or Mammalian Sterile 20-like kinase 3 is a member of the germinal center kinase-III family. MST3 contains a conserved kinase domain at its NH(2)-terminus and a regulatory domain at its COOH-terminus. Caspase-mediated cleavage of the regulatory domain of MST3 activates its intrinsic kinase activity and leads to nuclear translocation. Expression of COOH-terminal truncated MST3 in cells results in DNA fragmentation and induction of apoptosis. It can inhibit cell migration in a fashion dependent on autophosphorylation and can regulate paxillin phosphorylation through tyrosine phosphatase PTP-PEST. Mitogen activated protein kinase cascades have been conserved throughout evolution. In mammals, these cascades allow responses to complex stimuli such as growth factors and inflammatory cytokines. In yeast, STK25 functions upstream of the MAPK cascade.

Specificity:

phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174) Polyclonal Antibody detects endogenous levels of phospho-MST4 + MST3 + STK25 protein only when phosphorylated at Thr178 + Thr190 + Thr174

Alternative names:

phospho-MST4+MST3+STK25(phospho T178 + T190 + T174); p-MST4 + MST3 + STK25 (phospho T178 + T190 + T174); Mammalian STE20 like protein kinase 3; Mammalian Ste20 like protein kinase 4; Mammalian sterile 20 like 4; MASK; mess1; MGC94619; MST 3; MST 4; MST; Mst2; MST3 and SOK1 related kinase; MST3; MST3B; OTTHUMP00000018592; OTTHUMP00000018593; Protein kinase homolog serine/threonine kinase 3 (Ste20 yeast homolog); RGD1563568; RP11-111L24.5; Serine/threonine kinase 24 (STE20 homolog yeast); Serine/threonine kinase 24; Serine/threonine kinase 25; Serine/threonine protein kinase 24; Serine/threonine protein kinase MASK; Serine/threonine protein kinase MST 4; Serine/threonine protein kinase MST4; SOK1; STE20; Ste20 like; Ste20 like kinase; STE20 like kinase 3; STE20 like kinase MST 4; STE20 like kinase MST3; STE20 like kinase MST4; Ste20 yeast homolog; Ste20/oxidant stress response kinase 1; Sterile 20 (oxidant stress response kinase 1); sterile 20 like kinase 3; Sterile 20/oxidant stress-response kinase 1; STK3; Yeast Sps1/Ste20 related kinase 1; YSK1.

Species reactivity:

Human,Mouse,Rat,Cow,Horse,Sheep,

Host:

Rabbit

Ig type:

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Immunogen:

KLH conjugated synthesised phosphopeptide derived from human MST4/MST3/STK25 around the phosphorylation site of Thr178/Thr190/Thr174

Purification & Purity:

affinity purified by Protein A

Molecular weight:

39 kD

Applications:

WB,

Usage:

WB=1:500-2000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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