Background:

Estrogen and progesterone receptor are members of a family of transcription factors that are regulated by the binding of their cognate ligands. The interaction of hormone-bound estrogen receptors with estrogen responsive elements(EREs) alters transcription of ERE-containing genes. The carboxy terminal region of the estrgen receptor contains the ligand binding domain, the amino terminus serves as the transactivation domain, and the DNA binding domain is centrally located. Two forms of estrogen receptor have been identified, ER alpha and ER beta. ER alpha and ER beta have been shown to be differentially activated by various ligands. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (hPR-A and hPR-B), which arise from alternative splicing. In most cells, hPR-B functions as a transcriptional activator of progesterone-responsive gene, whereas hPR-A function as a transcriptional inhibitor of all steroid hormone receptors.

Specificity:

Phospho-Estrogen Receptor alpha (Ser118) monoclonal antibody detects endogenous levels of Estrogen Receptor alpha protein only when phosphorylated at Ser118.

Alternative names:

Estrogen Receptor alpha(S118); Estrogen Receptor alpha (phospho S118); p-Estrogen Receptor alpha (phospho S118); ER (phospho S118); p-ER (phospho S118); ER alpha (Phospho-Ser118); ER alpha (Phospho-S118); Phospho-ER alpha (S118); ; Phospho-ER alpha (Ser118); p-ER alpha (Ser118); p-ER alpha (S118); Phospho-Estrogen Receptor alpha (S118); Phospho-Estrogen Receptor alpha (S118); Estradiol receptor; Estrogen receptor alpha; Estradiol Receptor-alpha; Estrogen Receptor 1; Atherosclerosis, susceptibility to, included; DKFZp686N23123; ER Alpha; ER; ER-alpha; ERalpha; ER[a]; Era; ESR; ESR1; ESR1_HUMAN; ESR2; ESRA; Estr; Estrogen receptor 1 (alpha); Estrogen resistance, included; HDL cholesterol, augmented response of, to hormone replacement, included; Myocardial infarction, susceptibility to, included; NR3A1; Nuclear receptor subfamily 3 group A member 1; OTTHUMP00000017718; OTTHUMP00000017719; RNESTROR.

Species reactivity:

Human, Mouse

Host:

Rabbit

Ig type:

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Immunogen:

KLH conjugated Synthesised phosphopeptide derived from human ER alpha around the phosphorylation site of Ser118:QL(p-S)PF

Purification & Purity:

affinity purified by Protein A

Molecular weight:

~66 kDa

Applications:

IHC-P

Usage:

IHC-P=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

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