Background:

Cyan Fluorescent Protein (CFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. The fluorescence of EYFP was detected with an excitation filter of 485 nm and an emission filter of 545 nm, and that of ECFP was detected with filters of 420 and 486 nm (Perkin Elmer), respectively. In the present reporter assay, EYFP and ECFP were selected as a reporter protein and an internal control for normalization of transfection, respectively.

Specificity:

ECFP-tag monoclonal antibody detects over-expressed or recombinant proteins containing the ECFP epitope tag.

Alternative names:

ECFPTag; ECFPTag; tagECFP; Cyan Fluorescent Protein; Enhanced Cyan Fluorescent Protein; ECFP-Tag.

Species reactivity:

ECFP-Tag

Host:

Mouse

Ig type:

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Immunogen:

Recombinant ECFP

Purification & Purity:

affinity purified by Protein G

Molecular weight:

N/A

Applications:

WB,

Usage:

WB=1:1000-5000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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