Background:

The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.

Specificity:

Strep-tag monoclonal antibody detects over-expressed or recombinant proteins containing the Strep epitope tag.

Alternative names:

Strep; Strep Tag II; Strep.Tag; Strep-Tag-II; Strep Tag-II; STREP II TAG.

Species reactivity:

Strep-Tag

Host:

Mouse

Ig type:

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Immunogen:

KLH conjugated synthetic peptide Strep-Tag

Purification & Purity:

affinity purified by Protein G

Molecular weight:

N/A

Applications:

WB,

Usage:

WB=1:1000-5000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com