Background:

The human endothelins represent a gene family comprised of endothelin-1, endothelin-2, and endothelin-3, also known as ET-1, ET-2, and ET-3. Endothelins can affect the central nervous system and neuronal excitability, and they elicit potent vasoconstrictor action. The two receptor subtypes responsible for inducing vasoconstriction and vasodilation, ETA and ETB, have different receptor affinities for ET-1, ET-2, and ET-3. Of the three isopeptides, ET-2 has the most potent vasoconstrictor activity (1). Biologically active ETs are proteolytically generated from a larger precursor, the big-endothelin, by action of the endothelin-converting enzyme (ECE) family. ET-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells.

Specificity:

EDN1 polyclonal antibody detects endogenous levels of EDN1 protein.

Alternative names:

Big endothelin 1; Big endothelin-1; EDN 1; EDN1; EDN1 protein; EDN1; Endothelin1; ET 1; ET-1; ET1; OTTHUMP00000039245; PPET 1; PPET1; Preproendothelin 1; Preproendothelin-1; Preproendothelin1

Species reactivity:

Human,Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to Human EDN1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 23 kDa

Applications:

WB

Usage:

WB 1:500 - 1:2000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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