Background:

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.

Specificity:

PERK (Phospho-Thr980) polyclonal antibody detects endogenous levels of PERK protein only when phosphorylated at Thr980.

Alternative names:

p-PERK(Thr980); PERK(Phospho Thr980); PERK(Phospho T980); HRI; HsPEK; Pancreatic eIF2-alpha kinase; PEK; PRKR like endoplasmic reticulum kinase; WRS; DKFZp781H1925; EC 2.7.11.1; EIF2AK3; Eukaryotic translation initiation factor 2 alpha kinase 3; Heme regulated EIF2 alpha kinase

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

KLH conjugated synthesised phosphopeptide derived from mouse PERK around the phosphorylation site of Thr980: H(p-T)GQ

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~119 kDa

Applications:

WB IHC IF ELISA

Usage:

WB 1:500 - 1:2000 IHC 1:100 - 1:500 IF 1:100 - 1:500 ELISA 1:5000 - 1:10000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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