Background:

IL-12, a heterodimeric cytokine composed of two disulfide-bonded glycoprotein subunits, p35 and p40, has pleiotrophic activities including stimulation of the proliferation of activated T and NK cells, induction of IFN-g production by PBMCs, enhancement of the lytic activity of NK/LAK cells and promotion of T-helper (Th) 1 cell responses. The T cell response to IL-12 is mediated through two receptor proteins, designated IL-12Rb-1 and IL-12Rb-2 (4). The genes encoding human IL-12Rb-1 and IL-12Rb-2 map to chromosomes 19p13.1 and 1p31.3-p31.2, respectively. Increased IL-12Rb-2 expression is crucial in regulating Th1 differentiation, whereas IL-12Rb-1 expression is less restricted. Inhibition of IL-12 activity may provide treatment for diseases associated with pathologic Th1 responses, such as multiple sclerosis or Crohn’s disease, while administration of recombinant IL-12 may aid in the treatment for allergic disorders and asthma.

Specificity:

IL12RB1 polyclonal antibody detects endogenous levels of IL12RB1 protein.

Alternative names:

Interleukin-12 receptor subunit beta-1; IL-12 receptor subunit beta-1; IL-12R subunit beta-1; IL-12R-beta-1; IL-12RB1; IL-12 receptor beta component; CD_antigen=CD212; IL12RB1; IL12R; IL12RB

Species reactivity:

Mouse, Rat

Host:

Rabbit

Ig type:

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Immunogen:

KLH conjugated synthetic peptide derived from mouse IL-12RB1:401-500/738

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 80 kDa

Applications:

WB IF IHC ELISA

Usage:

WB: 1:500~1:2000 IHC: 1:100~1:500 IF: 1:100~1:500 ELISA: 1:5000~1:10000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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