Background:

DSS-AHC critical region on the X chromosome protein 1 (DAX1) is an orphan nuclear receptor encoded by the nuclear receptor subfamily 0 group B member 1 (NR0B1) gene. DAX1 possesses an atypical DNA binding domain that allows it to form heterodimeric complexes with DNA binding partners and repress transcriptional activity. During development, DAX1 is important for establishment of the hypothalamic-pituitary-adrenal gonadal axis. The receptor is essential for development of several important hormone-producing organs that determine this axis, including the adrenal glands, pituitary, hypothalamus, and the male and female reproductive organs. Research studies suggest that DAX1 plays a role in maintenance of pluripotency in embryonic stem cells. Loss of DAX1 function through deletion or mutation results in adrenal insufficiency and hypogonadotropic hypogonadism, while duplication of the NR0B1 gene on the X-chromosome causes dosage-sensitive sex reversal.

Specificity:

NR0B1 polyclonal antibody detects endogenous levels of NR0B1 protein.

Alternative names:

Nuclear receptor subfamily 0 group B member 1; DSS-AHC critical region on the X chromosome protein 1; Nuclear receptor DAX-1; NR0B1; AHC, DAX1

Species reactivity:

Human,Pig

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Immunogen:

Synthetic peptide, corresponding to Human NR0B1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 48 kDa

Applications:

WB IF

Usage:

WB: 1:2000~1:5000 IF 1:50 - 1:100

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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