Background:

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. Beside its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively.

Specificity:

EphB1/2 polyclonal antibody detects endogenous levels of EphB1/2 protein.

Alternative names:

Eph receptor B1+Eph receptor B2; EPH B1; EPH B2; Eph tyrosine kinase 2; Eph tyrosine kinase 3; EPHB1; EPHB2; Ephrin receptor EphB1; Ephrin receptor EphB2; Ephrin type B receptor 1; Ephrin type B receptor 2; EPHT 2; EPHT 3; ERK; Hek 5; Hek 6; Tyrosine protein kinase receptor EPH2; Tyrosine protein kinase receptor EPH3; EPHB1_HUMAN

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Immunogen:

KLH conjugated synthetic peptide derived from human EphB1+EphB2: 901-984/984

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 125 kDa

Applications:

WB IF IHC ELISA

Usage:

WB: 1:500~1:2000 IHC: 1:100~1:500 IF: 1:100~1:500 ELISA: 1:5000~1:10000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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