Background:

Desmosomes are a class of intracellular junction that tightly link adjacent cells in mechanically stressed tissues such as the epithelium and myocardium . They derive their characteristic strength from the protein Desmoplakin, which acts as a tether by binding the cytoplasmic component of the Desmosome at it’s N-terminus while its C-terminus is anchored to the intermediate-filament cytoskeleton . This association mitigates the impact of mechanical forces on the desmosome by distributing them throughout the cytoskeleton and tissue . Desmoplakin is essential for normal desmosomal adhesion ; defects can result in pathologies that include cardiomyopathy , keratoderma , or the skin blistering disease Epidermolysis bullosa . Phospho-Desmoplakin (Ser165/166) Antibody is directed against a site that was identified at Cell Signaling Technology (CST) using PTMScan®, our LC-MS/MS platform for modification site discovery. Please visit PhosphoSitePlus®, a modification site knowledgebase developed and maintained by CST, at www.phosphosite.org for more information.

Specificity:

Recognizes endogenous levels of Desmoplakin protein.

Alternative names:

Desmoplakin; DP; 250/210 kDa paraneoplastic pemphigus antigen

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Immunogen:

KLH-conjugated synthetic peptide of human Desmoplakin

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 331 kDa

Applications:

WB, IHC

Usage:

WB (1/500 - 1/2000), IHC (1/50 - 1/200)

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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