HLA-A polyclonal antibody BS67311
Specifications
| 50ul/100ul |
Background:
Antigen-presenting major histocompatibility complex class I (MHCI) molecule. In complex with B2M/beta 2 microglobulin displays primarily viral and tumor-derived peptides on antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR) on HLA-A-restricted CD8-positive T cells, guiding antigen-specific T cell immune response to eliminate infected or transformed cells.May also present self-peptides derived from the signal sequence of secreted or membrane proteins, although T cells specific for these peptides are usually inactivated to prevent autoreactivity.Both the peptide and the MHC molecule are recognized by TCR, the peptide is responsible for the fine specificity of antigen recognition and MHC residues account for the MHC restriction of T cells.Typically presents intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via IFNG-induced immunoproteasome or via endopeptidase IDE/insulin-degrading enzyme.Can bind different peptides containing allele-specific binding motifs, which are mainly defined by anchor residues at position 2 and 9.
Specificity:
Recognizes endogenous levels of HLA-A protein.
Alternative names:
HLAA; HLA class I histocompatibility antigen, A-1 alpha chain; MHC class I antigen A*1
Species reactivity:
Human, Mouse, Rat
Host:
Rabbit
Ig type:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen:
Recombinant full length protein of human HLA-A
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Molecular weight:
~ 42 kDa
Applications:
WB, IHC, IF/ICC
Usage:
WB (1/500 - 1/2000), IHC(1/50 - 1/200), IF/ICC (1/50 - 1/200)
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Precautions:
For research use only, not for use in diagnostic procedure.
More info:
Email: info@sobekbio.com
Orders:
Email: orders@sobekbio.com