Background:

The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses. The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N). Envelope protein is transcribed and translated from nested subgenomic RNA of the SARS-CoV-2 genome. Despite the SARS-CoV-2 envelope protein being very small, it possesses a number of functionalities that are critical for viral assembly and release. It is also a major virulence factor, a viroporin that contributes to the host immune mediated cytokine storm. A C-terminal motif on envelope protein binds to and mislocates a number of cell tight junction proteins in lung epithelial cells which may contribute to alveolar damage and acute respiratory distress syndrome (ARDS).

Specificity:

Recognizes COVID-19 Envelope Protein.

Alternative names:

Envelope small membrane protein; E protein; sM protein

Species reactivity:

COVID-19

Host:

Rabbit

Ig type:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of COVID-19 Envelope Protein. The exact sequence is proprietary.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 27 kDa

Applications:

ELISA, WB

Usage:

WB (1/1000 - 1/3000), ELISA: Use at an assay dependent dilution

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com