Background:

The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses. The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N). The nucleocapsid (N) protein is a phosphoprotein that plays a critical role in viral assembly. It binds to the viral genomic RNA, enabling its packaging into the ribonucleoprotein complex (RNP), and is the most abundant protein in the virion. Evidence from studies in SARS-CoV-2 and other coronaviruses suggest functions for nucleocapsid (N) protein beyond viral RNA packaging, including roles in viral replication and suppressing host immune responses to viral infection.

Specificity:

Recognizes COVID-19 Nucleocapsid Protein.

Alternative names:

Nucleoprotein; Nucleocapsid protein; NC; Protein N

Species reactivity:

COVID-19

Host:

Rabbit

Ig type:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of COVID-19 Nucleocapsid Protein. The exact sequence is proprietary.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 55 kDa

Applications:

ELISA, WB

Usage:

WB (1/1000 - 1/3000), ELISA: Use at an assay dependent dilution

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com