Background:

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments . Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers . Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases .

Specificity:

Recognizes endogenous levels of Cytokeratin 5 protein.

Alternative names:

Keratin type II cytoskeletal 5; 58 kDa cytokeratin; Cytokeratin-5; CK-5; Keratin-5; K5; Type-II keratin Kb5

Species reactivity:

Human

Host:

Mouse

Ig type:

Mouse IgG2a. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within human Cytokeratin 5. The exact sequence is proprietary.

Purification & Purity:

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 62 kDa

Applications:

WB, IHC

Usage:

WB (1/500 - 1/1000), IHC (1/100 - 1/300)

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com