SOX9 monoclonal antibody MB66135
Specifications
| 50ul/100ul |
Background:
Sox9 is a transcription factor with an HMG-box DNA binding domain that has homology to the HMG domain of the mammalian testis-determining factor, SRY . Sox9 regulates several important processes during embryonic development including chondrogenesis, during which it contributes to skeletal formation and digit specification . Sox9 also coordinates with steroidogenic factor-1 to direct Sertoli cell-specific expression of anti-Mullerian hormone during embryogenesis, thereby contributing to male sex determination . In addition, Sox9 is reportedly involved in the maintenance of adult stem cell populations, including multipotent neural stem cells , hair follicle stem cells , and mammary stem cells . Recent interest has focused on the role of Sox9 in tumor biology. For example, research studies have shown that Sox9 expression in lung adenocarcinoma induces a mesenchymal phenotype in tumor cells. Other research studies have shown that YAP1 induced upregulation of Sox9 confers cancer stem cell like properties on esophageal cancer cells . Moreover, Sox9 expression has been linked with several other tumor types including ovarian, prostate, and pancreatic malignancies .
Specificity:
Recognizes endogenous levels of SOX9 protein.
Alternative names:
Transcription factor SOX-9
Species reactivity:
Human
Host:
Mouse
Ig type:
Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Immunogen:
KLH-conjugated synthetic peptide encompassing a sequence within human SOX9. The exact sequence is proprietary.
Purification & Purity:
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Molecular weight:
~ 70 kDa
Applications:
WB, IHC
Usage:
WB (1/500 - 1/1000), IHC (1/100 - 1/300)
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Precautions:
For research use only, not for use in diagnostic procedure.
More info:
Email: info@sobekbio.com
Orders:
Email: orders@sobekbio.com