Background:

Tryptase is the most abundant neutral serine protease expressed in the secretory granules of all human mast cells. Tryptase is secreted upon the coupled activation-degranulation response of mast cells in peripheral tissues to physical factors such as trauma, toxins, venoms, endogenous mediators, and immune mechanisms (IgE-dependent and IgE-independent). Tryptase has distinct enzymatic functions that depend on the monomeric or homotetrameric state of this protein, the pH of the environment, and the presence or absence of heparin. Tryptase has the ability to cleave extracellular substrates such as vasoactive intestinal peptides, calcitonin gene-related peptides, fibronectins, fibrinogens, and kininogens. Tryptase is also a potent growth factor for epithelial cells, airway smooth muscle cells, and fibroblasts.

Specificity:

Recognizes endogenous levels of TPSAB1 protein.

Alternative names:

TPS1; TPS2; TPSB1; Tryptase alpha/beta-1; Tryptase-1; Tryptase I; Tryptase alpha-1

Species reactivity:

Human

Host:

Mouse

Ig type:

Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within human TPSAB1. The exact sequence is proprietary.

Purification & Purity:

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 30 kDa

Applications:

IHC

Usage:

IHC (1/100 - 1/300)

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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