Background:

Caldesmon-1 is an actin filament stabilizing protein involved in the regulation of cell contraction. Binding of caldesmon-1 to actin is weakened by phosphorylation and by calmodulin in the presence of calcium. Caldesmon-1 is encoded by a single gene, which is spliced to generate a widely distributed low molecular weight form and a smooth muscle specific high molecular weight form. Caldesmon-1 is phosphorylated by the cyclin dependent kinase cdc2 and Erk1/2 MAP kinase, both of which prevent the activity of caldesmon-1. Phosphorylation of caldesmon-1 by cdc2 is required for passage of cells through mitosis. Phosphorylation by Erk1/2 is important in regulating smooth muscle contraction. Caldesmon-1 activity may play a role in the formation of podosomes, adhesion complexes associated with the secretion of matrix metalloproteases, invasion, and metastasis.

Specificity:

Recognizes endogenous levels of Caldesmon protein.

Alternative names:

CAD; CDM; Caldesmon; CDM

Species reactivity:

Human

Host:

Mouse

Ig type:

Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within human Caldesmon. The exact sequence is proprietary.

Purification & Purity:

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 70 kDa

Applications:

WB, IHC

Usage:

WB (1/500 - 1/1000), IHC (1/100 - 1/300)

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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