Background:

Insulin degrading enzyme (IDE), initiates the cleavage of Insulin, resulting in Insulin response and resistance. However, IDE also degrades a variety of bioactive peptides, including Amyloid-β peptides, implicating IDE in certain age-related changes seen in Alzheimer’s disease. Studies show that when the expression of the IDE gene (chromosome 10q23.33) is altered, changes occur not only in glucose homeostasis, but also in the levels of brain Aβ40 and Aβ42 peptides. An IDE inhibitor, bacitracin, inhibits degradation of both Insulin and amylin, indicating that both are degraded through a common proteolytic pathway. Variations in the rate of proteolysis suggest that the function of IDE exhibits conformational dependence, which may lead to possible therapeutic interventions for diabetes, AD, and other diseases associated with IDE substrate proteolysis.

Specificity:

Recognizes endogenous levels of IDE protein.

Alternative names:

Insulin-degrading enzyme; Abeta-degrading protease; Insulin protease; Insulinase; Insulysin

Species reactivity:

Human

Host:

Mouse

Ig type:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence of human IDE. The exact sequence is proprietary.

Purification & Purity:

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 118 kDa

Applications:

WB

Usage:

WB (1/1000 - 1/2000)

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

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