Background:

Fumarate hydratase (Fumarase, FH) is canonically regarded as a mitochondrial enzyme of the tricarboxylic acid cycle that catalyzes fumarate to malate. Studies have demonstrated that deficiencies in FH can lead to hereditary leiomyomatosis and renal cell cancer or, in homozygous germline mutations, severe neurological and muscular disorders. There also exists a cytosolic fumarase isoenzyme that is involved in DNA damage repair.

Specificity:

Recognizes endogenous levels of Fumarase protein.

Alternative names:

Fumarate hydratase mitochondrial; Fumarase

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Ig type:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence of human Fumarase. The exact sequence is proprietary.

Purification & Purity:

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 50 kDa

Applications:

WB, IF/ICC

Usage:

WB (1/1000 - 1/3000), IF/ICC (1/100 - 1/200)

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com