Background:

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.

Specificity:

Recognizes the FLAG-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Alternative names:

DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag

Species reactivity:

N/A

Host:

Rabbit

Ig type:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Immunogen:

KLH-conjugated synthetic peptide DYKDDDDK.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

N/A

Applications:

WB, IP

Usage:

WB (1/1000 - 1/2000), IP (1/10 - 1/100)

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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