Background:

Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.

Specificity:

Recognizes endogenous levels of FOXO4 with a phosphorylation site at S262 protein.

Alternative names:

AFX; AFX1; MLLT7; Forkhead box protein O4; Fork head domain transcription factor AFX1

Species reactivity:

Human,Rat,Mouse

Host:

Rabbit

Ig type:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FOXO4 with a phosphorylation site at S262. The exact sequence is proprietary.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 157% (by SDS-PAGE).

Molecular weight:

~ 70 kDa

Applications:

WB, IHC

Usage:

WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com