Background:

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ16 may be involved in the regulation of fluid and pH balance. In the kidney, together with KCNJ10, mediates basolateral K+ recycling in distal tubules; this process is critical for Na+ reabsorption at the tubules (PubMed:24561201).

Specificity:

Recognizes endogenous levels of Kir5 protein.1 with a phosphorylation site at S416 protein.

Alternative names:

KCNJ16; Inward rectifier potassium channel 16; Inward rectifier K(+) channel Kir5.1; Potassium channel inwardly rectifying subfamily J member 16

Species reactivity:

Human,Rat,Mouse

Host:

Rabbit

Ig type:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Kir5.1 with a phosphorylation site at S416. The exact sequence is proprietary.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 156% (by SDS-PAGE).

Molecular weight:

~ 55 kDa

Applications:

WB, IHC, IF/IC

Usage:

WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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