Background:

Complement component 4-binding protein (C4BP) is a plasma glycoprotein that inhibits the classical pathway of complement activation, which is mediated through antibody targeting of foreign antigen. Structurally, C4BP is a disulfide linked, multimeric protein that is composed of seven α chains and one β chain. C4BP functions as a cofactor for C3β inactivator in the cleavage of C3β, and accelerates the decay of C4βC2α (C3 convertase) by acting as a cofactor in the cleavage of C4β by factor I. Streptococcal strains that express Ig-binding cell surface molecules, which are members of the M protein family,can bind to overlapping C4β binding sites in C4BP and therefore, interfere with the classical pathway of complement activation. Bacteria-bound C4BP may be an evolved mechanism that downregulates complement activation in the bacterial host microenvironment, thereby reducing the occurances of bacterial opsonization and phagocytosis.

Specificity:

C4BPB polyclonal antibody detects endogenous levels of C4BPB protein.

Alternative names:

C4b-binding protein beta chain; C4BPB

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to Human C4BPB.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 45 kDa

Applications:

WB IHC IF

Usage:

WB: 1:500~1:2000 IHC: 1:50~1:200 IF: 1:50~1:100

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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