Background:

WD-repeats are motifs that are found in a variety of proteins and are characterized by a conserved core of 40-60 amino acids, which commonly form a tertiary propeller structure. While proteins that contain WD-repeats participate in a wide range of cellular functions, they are generally involved in regulatory mechanisms involving signal transduction, apoptosis, transcriptional regulation and cell cycle control. WD repeats serve as sites for protein-protein interaction and some seem to mediate the assembly of protein complexes. With eight WD repeats, WDR48 (WD repeat-containing protein 48), also known as USP1-associated factor 1 and p80, is a 677 amino acid protein that functions to regulate deubiquitinating complexes via activation of USP1, USP12 and USP46. WDR48 enhances deubiquitination by increasing catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate. WDR48 is ubiquitously expressed and is mainly localized to the cytoplasm. There are five isoforms of WDR48 that are expressed as a result of alternative splicing events.

Specificity:

WDR48 polyclonal antibody detects endogenous levels of WDR48 protein.

Alternative names:

P80; UAF1; SPG60;

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein of human WDR48.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 76 kDa

Applications:

WB IHC

Usage:

WB 1:500 - 1:2000 IHC 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

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