Background:

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit.

Specificity:

Actived-Caspase-9 p35 (D315) polyclonal antibody detects detects endogenous levels of the 35 kDa large fragment of caspase-9 following cleavage at aspartic acid 315.

Alternative names:

APAF 3,APAF-3,APAF3,Apoptosis related cysteine peptidase,Apoptotic protease activating factor 3,Apoptotic protease Mch 6,Apoptotic protease Mch-6,Apoptotic protease-activating factor 3,CASP 9,CASP-9,CASP9, Caspase 9 apoptosis related cysteine peptidase,Caspase 9 Dominant Negative,Caspase 9c,Caspase-9, Caspase9,ICE LAP6,ICE like apoptotic protease 6,ICE-LAP6,ICE-like apoptotic protease 6,MCH6,RNCASP9

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 300-350 of Human Caspase 9.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 35 kDa

Applications:

WB IHC

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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