Background:

HCF-1 is a cellular protein required by VP16, a viral tegument, to activate the herpes simplex virus (HSV) immediate-early genes. In addition to playing an essential role in cell proliferation, HCF-1 also functions as a coactivator for the basic leucine zipper transcription factor LZIP (also designated Luman or CREB3 . Both LZIP and VP16 contain the binding motif (D/E) HXY (S/A), which is recognized by an amino terminal beta-propeller domain in HCF-1. LZIP, a member of the ATF/CREB family, is a type II membrane-associated glycoprotein that is ubiquitously expressed in adult and fetal tissues. LZIP associates with the endoplasmic reticulum, where it sequesters most of the cellular HCF-1. Like other CREB/ATF family members, LZIP activates transcription from genes containing cyclic AMP response elements (CREs) . LZIP activity is repressed by the inhibitory interaction of HCLP-1 .

Specificity:

CREB3 polyclonal antibody detects endogenous levels of CREB3 protein.

Alternative names:

Cyclic AMP-responsive element-binding protein 3; CREB-3; cAMP-responsive element-binding protein 3; Leucine zipper protein; Luman; Transcription factor LZIP-alpha; CREB3; LZIP

Species reactivity:

Human,Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human CREB3.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 44 kDa

Applications:

WB IF

Usage:

WB: 1:500~1:2000 IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com