Background:

Patients with polycythemia vera (PV), a neoplastic stem cell disorder that leads to excessive production of all myeloid cell lines, overexpress the cell surface antigen CD177, also designated NB1. The increased output, especially of red blood cells, increases whole blood viscosity and causes vascular occlusion and ischemia. Because of the marked upregulation of CD177 in PV patients, as compared to healthy individuals or those with other erythrocytosis-related conditions, analysis of this protein presents a useful tool for diagnosis and research into the mechanisms of PV.

Specificity:

CD177 polyclonal antibody detects endogenous levels of CD177 protein.

Alternative names:

CD177 antigen; Human neutrophil alloantigen 2a; HNA-2a; NB1 glycoprotein; NB1 GP; Polycythemia rubra vera protein 1; PRV-1; CD177; CD177; NB1; PRV1; UNQ595; PRO1181

Species reactivity:

Human,Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human CD177.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 46 kDa

Applications:

WB

Usage:

WB 1:500 - 1:2000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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