Background:

The B7-related cell surface proteins CD80 (B7-1) and CD86 (B7-2) are expressed on antigen presenting cells. CD80 and CD86 bind to the homologous T cell receptors CD28 and CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31% overall amino acid identity with CD28 and it has been proposed that CD28 and CTLA-4 are functionally redundant. ICOS (inducible co-stimulator) is related to CD28 and CTLA-4, and these three proteins are thought to compose a receptor family. ICOS stimulation enhances T cell responses and superinduces the synthesis of IL-10, but it does not induce IL-2 upregulation.

Specificity:

ICOS polyclonal antibody detects endogenous levels of ICOS protein.

Alternative names:

Inducible T-cell costimulator; Activation-inducible lymphocyte immunomediatory molecule; CD278; ICOS; AILIM

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human ICOS.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 22 kDa

Applications:

WB,IF

Usage:

WB 1:500 - 1:2000 IF 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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