Background:

Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an a heavy chain that contains three subdomains (α1, α2, α3), and a non-covalent associating light chain, known as β-2-Microglobulin. β-2-Microglobulin associates with the a3 subdomain of the a heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The α1 and α2 domains of the a heavy chain form the peptide antigen-binding cleft.

Specificity:

B2M polyclonal antibody detects endogenous levels of B2M protein.

Alternative names:

B2MG; Beta 2 microglobin; Beta 2 microglobulin; Beta 2 microglobulin precursor; Beta chain of mhc class 1 proteins; Beta chain of MHC class I molecules; Beta-2-microglobulin form pI 5.3; CDABP0092; Hdcma22p;

Species reactivity:

Human,Mouse,Rat,Monkey

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human B2M.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 13 kDa

Applications:

WB,IHC

Usage:

WB 1:2000 - 1:5000 IHC 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com