Background:

A large number of low molecular weight GTP-binding proteins of the Ras superfamily have been identified in eukaryotic cells; they regulate many fundamental processes such as cell growth, vesicle traffic and cytoskeletal organization. GTPase-activating proteins accelerate the intrinsic rate of GTP hydrolysis of Ras-related proteins, resulting in downregulation of their active form. Rac 1 is activated in a type I interferon (IFN) dependent manner; its function is required for downstream engagement of the p38 MAP kinase pathway. The p38 MAP kinase plays an essential role in IFN-dependent transcriptional regulation. The serine/threonine kinase Akt, of the phospho-inositide 3-kinase signal transduction pathway phosphorylates serine 71 of Rac 1.

Specificity:

P-RAC1(S71) polyclonal antibody detects endogenous levels of RAC1 protein only when phosphorylated at Ser71.

Alternative names:

Ras-related C3 botulinum toxin substrate 1; Cell migration-inducing gene 5 protein; Ras-like protein TC25; p21-Rac1; RAC1; TC25; MIG5

Species reactivity:

Human,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic phosphopeptide derived from human RAC1 around the phosphorylation site of Serine 71.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 23 kDa

Applications:

WB

Usage:

WB: 1:500~1:2000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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