Background:

Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for protein synthesis within the mitochondrion. The mitoribosomes are composed of a 4:1 ratio of protein to RNA, with the proteins forming two subunits, the 28S subunit and the 39S subunit. Across species, the proteins that make up the mitoribosome subunits vary greatly in sequence, preventing easy recognition by sequence homology. MRP-L15 (mitochondrial 39S ribosomal protein L15), also known as L15mt, is a 296 amino acid mitochondrial ribosomal protein belonging to the ribosomal protein L15P family. Localized to mitochondria, MRP-L15 is present in the 39S subunit of the mitoribosomes.

Specificity:

MRPL15 polyclonal antibody detects endogenous levels of MRPL15 protein.

Alternative names:

39S ribosomal protein L15, mitochondrial; L15mt; MRP-L15

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 191-235 of Human MRPL15.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).

Molecular weight:

~ 36 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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