Background:

Phosphatidylinositol-glycans (PIGs) are multi-pass transmembrane proteins that localize to the endoplasmic reticulum. PIGs exhibit various functions, but all are crucial for the biosynthesis of the glycosylphosphatidylinositol (GPI)-anchor. Some PIG proteins are components of the GPI transamidase complex and play a role in the recognition of either the GPI attachment signal or the lipid portion of GPI. Other PIGs belong to the glycosyltransferase complex and function in the transfer of N-acetylglucosamine (GlcNAc) to phosphatidylinositol (PI). A variety of other PIGs play distinct roles in GPI synthesis including mannosylation of the GPI-anchor. PIG-W (Phosphatidylinositol-glycan biosynthesis class W protein) is a 504 amino acid multi-pass membrane protein that functions in the third step of GPI biosynthesis and acylates the inositol ring of phosphatidylinositol.

Specificity:

PIGW polyclonal antibody detects endogenous levels of PIGW protein.

Alternative names:

Phosphatidylinositol-glycan biosynthesis class W protein (EC:2.3.-.-); PIG-W; PIGW

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

A synthetic peptide corresponding to residues in Human PIGW.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 57 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -24°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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