Background:

Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures, and are crucial components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer of two heavy chains (MHC) and four light chains (MLC) that interact with Actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. MYH3 (Myosin heavy chain 3), also known as muscle embryonic Myosin heavy chain or SMHCE, is a 1,940 amino acid that localizes to the thick filaments of myofibrils. While highly expressed in fetal skeletal muscle, MYH3 is barely detectable in adult skeletal muscle. Defects in the gene encoding MYH3, which maps to human chromosome 17p13.1, are the cause of distal arthrogryposis type 2B (DA2B).

Specificity:

MYH8 polyclonal antibody detects endogenous levels of MYH8 protein.

Alternative names:

Myosin-8; Myosin heavy chain 8; Myosin heavy chain, skeletal muscle, perinatal; MyHC-perinatal; MYH8

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

A synthetic peptide corresponding to residues in Human MYH8.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 223 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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