Background:

TAF1L (transcription initiation factor TFIID 210 kDa subunit) is a 1,826 amino acid protein that serves as a functional substitute for TAF II p250 in spermatogenic cells. In somatic cells, TAF II p250, which is located on the X chromosome, is a general transcription factor which initiates preinitiation complex assembly through direct interaction with the TATA promoter element. However, during male meiosis, sex chromosomes are transcriptionally silenced, inactivating the transcriptional activity of TAF II p250. With an autosomal location, TAF1L functionally replaces TAF II p250 in testes. It is documented that the gene encoding human TAF1L arose by retroposition of TAF II p250 mRNA during primate evolution after species divergence.

Specificity:

TAF1 pAb detects endogenous levels of TAF1 protein.

Alternative names:

Transcription initiation factor TFIID subunit 1; Cell cycle gene 1 protein; TBP-associated factor 250 kDa; p250; Transcription initiation factor TFIID 250 kDa subunit; TAF(II)250; TAFII-250; TAFII250; TAF1; BA2R; CCG1; CCGS; TAF2A

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding Human TAF1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 310 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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