Background:

α-2-Macroglobulin (α-2M) is a homotetrameric serum protein consisting of four identical subunits that form dimers through disulfide bonds. Initially, α-2M was characterized as a pan-proteinase inhibitor thatwas able to “bait” proteinases into cleaving specific peptide sequences on α-2M. This interaction induces a conformational change in α-2M, thus enabling it to “trap”the proteinase and inhibit its further activity. Subsequently, α-2M has also been shown to function as a carrier protein and regulator of cytokines during inflammation. Circulating transforming growth factor β (TGFβ) in serumis primarily bound to α-2M,which renders TGFβ inactive. α-2M also binds to IL-6 and, thereby, increasesthe concentration of IL-6 near lymphocytes, hepatocytes and stem cells involved inmediating the inflammatory cascade. Mutations and deletions in the gene encoding α-2M are associated with an increased incidence of Alzheimer’s Disease (AD),which isconsistent with the role of α-2M inmediating the clearance and degradation of Aβ, the major component of β-Amyloid deposits accumulated during AD.

Specificity:

A2M polyclonal antibody detects endogenous levels of A2M protein.

Alternative names:

Alpha-2-macroglobulin; Alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; A2M; CPAMD5

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

A synthetic peptide corresponding to residues in Human A2M.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 185 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:203

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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