Background:

RAIN, also known as RASIP1 (Ras-interacting protein 1), is a 963 amino acid protein that contains one dilute domain and one Ras-associating domain. The RAIN protein is highly expressed in heart and detected at lower levels in placenta and pancreas. RAIN interacts with H-Ras, Rap 1A, Rap 2, R-Ras, Raf-1 and TC 21, as well as with Ras family members that have been activated by GTP binding. RAIN interacts with Ras in a GTP-dependent manner in vitro and in vivo, requiring an intact Ras core effector-binding domain for this interaction, and thus fits the definition of a Ras effector. Unlike other Ras effectors, RAIN localizes to the perinuclear, juxta-Golgi vesicles in intact cells and is recruited to the Golgi by activated Ras. Containing twelve exons, the RAIN gene is conserved in dog, cow, mouse, rat, chicken and zebrafish, and maps to human chromosome 19q13.33.

Specificity:

RASIP1 polyclonal antibody detects endogenous levels of RASIP1 protein.

Alternative names:

Ras-interacting protein 1; Rain; RASIP1

Species reactivity:

Human,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

A synthetic peptide corresponding to residues in Human RASIP1

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 103 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

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