Background:

Proteins expressed in the endoplasmic reticulum (ER) are subjected to a tight quality control. Terminally misfolded proteins in the endoplasmic reticulum (ER) are retrotranslocated to the cytoplasm and degraded by proteasomes through a mechanism known as ER-associated degradation (ERAD). EDEM (ER degradation-enhancing alpha-mannosidase-like) protein is a type II membrane protein that localizes to the ER and is directly involved in ERAD. EDEM targets misfolded glycoproteins for degradation in an N-glycan-dependent manner and extracts misfolded glycoproteins from the calnexin cycle. The human EDEM gene maps to chromosome 3p.

Specificity:

EDEM1 polyclonal antibody detects endogenous levels of EDEM1 protein.

Alternative names:

ER degradation-enhancing alpha-mannosidase-like protein 1; EDEM1; EDEM; KIAA0212

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

A synthetic peptide corresponding to residues in Human EDEM1

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 74 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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