Background:

Prostasin, a serine protease first identified in prostate tissue, activates epithelial sodium channels in a variety of tissues. Though typically a membrane-anchored protein, free prostasin is also found in physiologic fluids and tissue culture media, indicating a mechanism for secretion from the cells as well. Aprotinin and other protease inhibitors suppress the channel-activating capacity of prostasin, while aldosterone increases prostasin expression and stimulates sodium uptake. In addition, DNA methylation negatively correlates with prostasin expression in cancer cells, while enforced reexpression decreases invasiveness as well as metastatic potential, implying that prostasin activity reflects epithelial cell physiology.

Specificity:

PRSS8 polyclonal antibody detects endogenous levels of PRSS8 protein.

Alternative names:

Prostasin; Channel-activating protease 1; CAP1; Serine protease 8; PRSS8

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

A synthetic peptide corresponding to residues in Human PRSS8

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 36 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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