Background:

Steroid receptor RNA activator (SRA) selectively mediates transactivation of steroid hormone receptors. Specifically, SRA exists as both an RNA transcript that forms a complex with steroid receptor coactivator-1 and as a stably expressed protein. There are six RNA motifs in SRA that are important for coactivation. SRA is ubiquitously expressed in normal tissues with higher levels of expression in liver and skeletal muscle. SRA is expressed at a low level in brain . SRA is expressed at higher levels in breast tumor than in normal tissue. Overexpression of SRA stimulates ERalpha transcriptional activity. In cells transfected with antisense oligodeoxynucleotides to SRA, ERalpha expression is reduced in a dose-dependent fashion . SMRT/HDAC1 associated repressor protein (SHARP) binds to SRA and inhibits SRA-potentiated steroid receptor transciption.

Specificity:

SRA1 polyclonal antibody detects endogenous levels of SRA1 protein.

Alternative names:

Steroid receptor RNA activator 1; Steroid receptor RNA activator protein; SRAP; SRA1; PP7684

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

A synthetic peptide corresponding to residues in Human SRA1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 26 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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