Background:

Med31 (mediator of RNA polymerase II transcription subunit 31), also known as Soh1 or CGI-125, is a 131 amino acid protein that belongs to the mediator complex subunit 31 family. Mediator proteins serve as connectors between transcriptional activators and basal transcription machinery. Med31 is an evolutionary conserved coregulator of RNA polymerase II transcription and is a stable component of the Mediator complex. It is involved in forming a scaffold with other regulatory proteins for the assembly of a functional preinitiation complex with RNA polymerase II and general transcription factors. Med31 is required for normal levels of gene conversion during meiosis and contributes in the coordination of cellular processes by regulating the expression of larger sets of genes.

Specificity:

MED31 polyclonal antibody detects endogenous levels of MED31 protein.

Alternative names:

Mediator of RNA polymerase II transcription subunit 31; Mediator complex subunit 31; Mediator complex subunit SOH1; hSOH1; MED31; SOH1; CGI-125

Species reactivity:

Human,Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

A synthetic peptide corresponding to residues in Human MED31.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 16 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

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