Background:

Endonuclease G (ENDOG), a nuclear encoded protein, localizes to the mitochondria (1). This sugar-nonspecific nuclease, responsible for major mitochondrial nuclease activity, preferentially cleaves single-stranded DNA(ssDNA). Synthesized as a propeptide with an amino-terminal presequence that targets the nuclease to mitochondria, ENDOG translocates to neuclei on apoptotic stimulation and act as a nuclease without sequence specificity. Both exonucleases and DNase I stimulate the ability of ENDOG to generate double-stranded DNA cleavage products at physiological ionic strengths, suggesting that these activities work in concert with ENDOG in apoptotic cells to ensure efficient DNA breakdown.

Specificity:

ENDOG polyclonal antibody detects endogenous levels of ENDOG protein.

Alternative names:

Endonuclease G, mitochondrial; Endo G

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

A synthetic peptide corresponding to residues in Human ENDOG.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 35 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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