Background:

Lamellipodin, also called ras-associated and pleckstrin homology domains-containing protein 1 (RAPH1), is a 1302 amino acid member of the MRL family. The peripheral membrane protein mediates localized membrane signals and colocalizes at the tips of filopodia and lamellipodia with ENAH/VASP. Aside from the main isoform, nine additional isoforms have been identified for Lamellipodin (RMO1, RMO1a, RMO1b, RMO1c, RMO1ab, RMO1ac, RMO1bc, RMO1abc and RMO1-RAPH1). RMO1-RAPH1 is expressed in a wide variety of tissues, most highly in brain, heart, ovary and developing embryo. RMO1 is also widely expressed, with highest amounts in liver. Lamellipodin is down regulated in breast and ovarian cancers and shows reduced expression in metastatic osteosarcomas in comparison to primary osteosarcoma tumors.

Specificity:

RAPH1 polyclonal antibody detects endogenous levels of RAPH1 protein.

Alternative names:

Ras-associated and pleckstrin homology domains-containing protein 1; RAPH1; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein; Lamellipodin; Proline-rich EVH1 ligand 2; PREL-2; Protein RMO1; RAPH1; ALS2CR18; ALS2CR9; KIAA1681; LPD; PREL2; RMO1

Species reactivity:

Human,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

A synthetic peptide corresponding to residues in Human RAPH1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 135 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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