Background:

ASAH3 (N-acylsphingosine amidohydrolase (alkaline ceramidase) 3), also designated ACER1, is a 264 amino acid multi-pass membrane protein that localizes to the membrane of the endoplasmic reticulum. Expressed predominately in epidermal tissue, ASAH3 catalyzes the hydrolysis of the sphingolipid ceramide into sphingosine and a free fatty acid, thereby playing an important role in the regulation of bioactive ceramide and sphingosine levels within the cell. ASAH3 functions at an optimal pH of 8.0 and has high specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine, but not D-ribo-phytosphingosine or D-erythro-dihydrosphingosine as a backbone.

Specificity:

ACER1 polyclonal antibody detects endogenous levels of ACER1 protein.

Alternative names:

Alkaline ceramidase 1; AlkCDase 1; Alkaline CDase 1; EC=3.5.1.23; Acylsphingosine deacylase 3; N-acylsphingosine amidohydrolase 3; ACER1; ASAH3

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

A synthetic peptide corresponding to residues in Human ACER1

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 50 kDa

Applications:

WB IHC

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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