Background:

HCCA2 (Hepatocellular carcinoma-associated gene 2), also known as MOB2 (Mps one binder kinase activator-like 2), is a 467 amino acid protein that belongs to the MOB1/phocein family. HCCA2 is expressed in lung, spleen, brain, and fetal liver. It is highly expressed in hepatocellular carcinoma. HCCA2 is localized in the perinuclear region of the cytoplasm in liver cancer tissues and colocalizes with MAD2L2 in the nucleus of Hela cells. HCCA2 is chracaterized by two N-glycosylation sites, six N-myristolylation sites, two Src homolgy 3 (SH3), and several phosphorylation motifs which indicate that this protein may play a role in an intracellular signal transduction cascade. HCCA2 binds to and regulates the autophosphorylation of the related human serine/threonine kinase 38 (NDR1) and serine/threonine kinase 38L (NDR2). It has been shown that HCCA2 plays a critical role in cell cycle regulation. Overexpression of the protein during the G0/G1 phase inhibits cell proliferation causing cell cycle arrest.

Specificity:

HCCA2 polyclonal antibody detects endogenous levels of HCCA2 protein.

Alternative names:

MOB kinase activator 2; Mob2 homolog; Mps one binder kinase activator-like 2; MOB2; HCCA2

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

A synthetic peptide corresponding to residues in Human HCCA2.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 28 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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