Background:

CD52 is a glycosylphosphatidylinositol (GPI)-linked surface antigen present at high levels on epithelial cells lining the male reproductive tract, thymocytes, lymphocytes, monocytes and macrophages. It is also present at variable levels on lymphoid malignancies. During sperm maturation, spermatozoa passing through the genital tract acquire CD52 that is shed from the epithelial cell lining into seminal plasma. CD52 is detectable on the surface of epididymal sperm and in the ejaculate but not on spermatogenetic cells or testicular spermatozoa. The peptide backbone of CD52, which consists of 12 amino acids, is considered a mere scaffold for posttranslational modifications, such as GPI-anchor and N-glycosylation.

Specificity:

CD52 polyclonal antibody detects endogenous levels of CD52 protein.

Alternative names:

CAMPATH-1 antigen; CDw52; Cambridge pathology 1 antigen; Epididymal secretory protein E5; Human epididymis-specific protein 5; He5; CD52; CD52; CDW52, HE5

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

A synthetic peptide corresponding to residues in Human CD52.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 15 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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