Background:

In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequencespecific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcriptional start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Human TFIIE consists of two subunits of 56 kDa and 34 kDa molecular weight, respectively. The structure of TFIIE appears to be a heterotetramer(α2 β2) both subunits being required for optimal basal-level transcription .

Specificity:

TFIIE-β polyclonal antibody detects endogenous levels of TFIIE-β protein.

Alternative names:

Transcription initiation factor IIE subunit beta; TFIIE-beta; General transcription factor IIE subunit 2; GTF2E2; TF2E2; TFIIE β; TFIIEβ;

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 193-238 of Human TFIIE-β.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 33 kDa

Applications:

WB IHC

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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