Background:

Upon activation, the small GTPase Rho D (also designated RhoHP1 and ARHD) contributes to rearrangement of the actin cytoskeleton and cell surface and also governs endosome motility and distribution. The effects of Rho D antagonize those of its family member, Rho A, by dissasembling actin stress fibers normally enhanced by Rho A. Additionally, Rho D disengages focal adhesions, resulting in retardation of cell migration. Accordingly, transfection of a constitutively active form of Rho D (designated Rho D G26V) reverses the invasive phenotype of Gαolf induced cells, implying the possiblity of a therapeutic use for activated Rho D in counteracting tumor metastasis.

Specificity:

Rho D polyclonal antibody detects endogenous levels of Rho D protein.

Alternative names:

Rho-related GTP-binding protein RhoD; Rho-related protein HP1; RhoHP1; RHOD; ARHD

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 156-201 of Human Rho D.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 23 kDa

Applications:

WB ICC IP

Usage:

WB: 1:500~1:1000 ICC: 1:50~1:200 IP: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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