Background:

SHH is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo.

Specificity:

sonic hedgehog

Alternative names:

HHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Ig type:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Immunogen:

Recombinant protein expressed in E.coli corresponding to amino acids 24-197 of human SHH

Purification & Purity:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Molecular weight:

27.6 kDa (Actual 22 kDa/55 kDa)(Predicted)

Applications:

IF, WB

Usage:

WB: 1:2000, IF (1:100)

Storage:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Precautions:

For research use only, not for use in diagnostic procedure.

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