Background:

The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. The protein exhibits antimicrobial activity against pathogenic bacteria. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. ECP is a potent cytotoxic protein capable of killing cells of guinea pig tracheal epithelium, mammalian leukemia, epidermis carcinoma, and breast carcinoma, as well as non-mammalian cells such as parasites, bacteria, and viruses. Mature ECP is cytotoxic to human bronchial epithelial (BEAS-2B) cells by specific binding to cell surface heparan sulfate proteoglycans (HSPGs) followed by endocytosis. ECP triggers apoptosis by caspase-8 activation through mitochondria-independent pathway. Increases in chromatin condensation, sub-G1 population, PARP cleavage, and DNA fragmentation indicate that ECP induces apoptosis in human bronchial epithelial (BEAS-2B) cells.

Specificity:

Ribonuclease 3 monoclonal antibody detects endogenous levels of Ribonuclease 3 protein.

Alternative names:

ECP, RNase 3, Cytotoxic ribonuclease, ECP, ECP, Eosinophil cationic protein, OTTHUMP00000164017, Ribonuclease 3, Ribonuclease, RNase A family, 3, RNase 3, RNASE3, RNS3 antibody

Species reactivity:

Human

Host:

Mouse

Ig type:

Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein within human Ribonuclease 3 aa 28-120.

Purification & Purity:

Protein G purified.

Molecular weight:

18 kDa

Applications:

WB ICC IHC FC

Usage:

WB:1:500-1:2000 ICC:1:100 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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