Background:

Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110α and p110β interact with p85α, and p110α has also been shown to interact with p85β in vitro. p110δ expression is restricted to white blood cells. It has been shown to bind p85α and β, but it apparently does not phosphorylate these subunits. p110δ seems to have the capacity to autophosphorylate. p110γ does not interact with the p85 subunits. It has been shown to be activated by α and βγ heterotrimeric G proteins.

Specificity:

PI3K p85α monoclonal antibody detects endogenous levels of PI3-kinase p85 subunit alpha protein.

Alternative names:

PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PIK3R1, GRB1, GRB1, p85 alpha, p85, P85A, Phosphatidylinositol 3 kinase associated p 85 alpha, Phosphatidylinositol 3 kinase regulatory 1, Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha), Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, Phosphatidylinositol 3-kinase regulatory subunit alpha, Phosphoinositide 3 kinase, regulatory subunit 1 (alpha), PI3 kinase p85 subunit alpha, PI3-kinase regulatory subunit alpha, PI3-kinase subunit p85-alpha, PI3K, PI3K regulatory subunit alpha, Pik3r1, PtdIns 3 kinase p85 alpha, PtdIns-3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit p85-alpha,

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Ig type:

Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein within human PI3-kinase p85 subunit alpha aa 1-200.

Purification & Purity:

ProG affinity purified.

Molecular weight:

84 kDa

Applications:

WB IHC ICC

Usage:

WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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